• Where do we fit?

    Higher Order Structure Characterization of Antibodies

    IonDx Biophysical Fingerprinting

    - Scan in seconds
    - Femtomole sensitivity
    - Orthogonal insights to protein higher order structure
    - mAbs, antibody-drug conjugates, polymers, lipoproteins, exosomes...

    Resource requirements: Large circle = lowest, small circle = highest

    Based on Weiss et al. Journal of Pharmaceutical Sciences 105 (2016) 3465-3470

    Technical Decision Making With Higher Order Structure Data: Perspectives on Higher Order Structure Characterization From the Biopharmaceutical Industry

  • 100% Electrospray Ion Mobility Focused

    Unlock an orthogonal method for HOS characterization

    Rapid Scans of mAb Higher Order Structure

    mAb HOS data in less than 2 minutes

    mAbs have fluctuating & dynamic higher order structure in solution, including the presence of oligomers. We measure the cross-sectional area of all those conformations in <2 min with femtomole sensitivity. Typical samples are 0.05 mg/ml and we consume 100 nl during a single scan. In this single scan plot you see the baseline resolution of the NISTmAb monomer and dimer.

    Investigate mAb Oligomers

    Quantitative direct measurement of macromolecules

    Ion mobility provides you with a rapid means of surveying not only intact mAbs, but their non-specific aggregates and other higher order structures formed in solution. Common applications include ADC characterization and forced degradation pathways. This plot reveals conformations of singly-charged NISTmAb monomers, dimers and trimers with increased concentration. The m/z of these singly-charged ions extends beyond 450,000 precluding detection by standard mass spectrometers.

  • Fenn introduced proteins into the gas phase.

    Then we argued about their conformation for 30 yrs.

    Finally, measurements not distorted by electrospray.

    We measure gas phase cross-sections of intact singly-charged macromolecules

    using our patented ion mobility spectrometer

     

    IT'S SIMPLE.

    IonDx electrospray ion mobility of antibody molecules for biosimilar characterization

    Our measurements cannot be made with any other instrument.

    We measure the size & shape (conformation) of giant biomolecules via a DIRECT ion mobility method that uses a combination of aerodynamics and electric fields.

  • Gas Phase Collisional Cross-Section of Proteins Varies by MW and Folding State

    IonDx measures SINGLY charged intact protein ions so we avoid the well-established correlation between charge state and protein unfolding in the gas phase, while making DIRECT measurements of protein collisional cross-sections.

  • Curious?

    You know you want to learn more.

    Native Electrospray Ion Mobility of your Macromolecules is at your fingertips.

    Download our recent poster from the 6th International Symposium on Higher Order Structure of Protein Therapeutics: "A Better Fingerprint for Therapeutic Protein Higher Order Structure"

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